ABSTRACT
Introducao: Asvacinas anti-Sars CoV-2 demonstraram que sao primordiais para o controle da pandemia por possibilitarem o desenvolvimento de imunidade em pessoas devidamente vacinadas. Objetivos: O objetivo do presente estudo foi avaliar o desenvolvimento de anticorpos neutralizantes em profissionais de saude apos duas doses das vacinasCoronaVac e AstraZeneca. Metodologia: Exames com principio de quimioluminescencia LIAISONSARS CoV-2 S1/S2 IgG - DIASORIN foram realizados no Laboratorio de Sorologia do Hemocentro de Joao Pessoa, e contagens acima de 15 AU/mL (Unidades Formadoras de Anticorpos por mL) caracterizaram desenvolvimento de protecao anti-Sars CoV-2. Cem (100) profissionais de saude foram estudados apos imunizacao com CoronaVac e AstraZeneca. Resultados: Apos a primeira dose da vacina CoronaVac, 61,4% dos profissisonais de saude ainda permaneceram com baixos niveis de anticorpos;apenas 38,6% conseguiram se imunizar. Ja apos a segunda dose da CoronaVac, apenas 12% permaneceram nao imunes, e 88% se imunizaram. Desses nao imunes, um profissional de saude faleceu com Covid-19.Considerando a vacina Astrazeneca, apenas 23,8% dos profissionais de saude nao se imunizaram apos a primeira dose, sendo que 76, 2% desenvolveram anticorpos neutralizantes. Apos receberem a segunda dose da Astrazeneca, 100% dos profissionais ficaram imunizados. Discussao: Considerando que, apos contato com a vacina, nosso organismo demora entre 7 - 14 dias para desenvolvimento de anticorpos, e que estes tem meia vida entre 20 a 28 dias, os soros dos participantes foram coletados apos exatos 20 dias a partir da imunizacao dos participantes para garantia de deteccao de anticorpos. Com o final da meia vida circulante dos anticorpos IgG, os linfocitos T de memoria serao os responsaveis pela resposta imune do nosso organismo. Conclusao: O presente estudo demonstrou a evolucao do desenvolvimento de anticorpos neutralizantes contra Covid-19 apos cada dose dos diferentes tipos de vacinas aplicados em profissionais de saude, bem como a importancia de mais de uma dose de imunizante para completa seguranca vacinal da populacao. Copyright © 2022
ABSTRACT
SARS-CoV-2 is the causative agent of COVID-19. The dimeric form of the viral Mpro is responsible for the cleavage of the viral polyprotein in 11 sites, including its own N and C-terminus. The lack of structural information for intermediary forms of Mpro is a setback for the understanding its self-maturation process. Herein, we used X-ray crystallography combined with biochemical data to characterize multiple forms of SARS-CoV-2 Mpro. For the immature form, we show that extra N-terminal residues caused conformational changes in the positioning of domain-three over the active site, hampering the dimerization and diminishing its activity. We propose that this form preludes the cis and trans-cleavage of N-terminal residues. Using fragment screening, we probe new cavities in this form which can be used to guide therapeutic development. Furthermore, we characterized a serine site-directed mutant of the Mpro bound to its endogenous N and C-terminal residues during dimeric association stage of the maturation process. We suggest this form is a transitional state during the C-terminal trans-cleavage. This data sheds light in the structural modifications of the SARS-CoV-2 main protease during its self-maturation process.
Subject(s)
Peptide Hydrolases/chemistry , Peptide Hydrolases/metabolism , SARS-CoV-2/metabolism , Viral Proteins/chemistry , Viral Proteins/metabolism , Catalytic Domain/physiology , Crystallography, X-Ray/methods , Dimerization , HumansABSTRACT
The objective of the research was to analyse the mobility and evolution of COVID-19 in the state of Amazonas, Brazil. For this, data were collected from confirmed cases of COVID-19 from the Fundacao de Vigilancia ern Sadde (FVS / AM), treated by the geostatistical technique of the Weighted Distance Inverse (IDW) in the geoprocessing software QGIS 3.4. These data were analysed statistically with the flows of riverboats in the State. The trips done to access health services at different levels of complexity in the State were also mapped from the IBGE Regic survey (REGIC, 2018). The results showed the association of the diffusion and evolution of the cases of COVID-19 with river transport by mobility on line riverboats, being "entry points" for the virus and with the movement of the disease toward the western part the state. Therefore, the spread by contagion in Amazonas followed the paths of the waters, by the intercity mobility of people and goods in river transport and the rapid evolution of the increase in the number of cases in the municipalities. The overall aim of this paper was to analyze the mobility and evolution of COVID-19 in Brazilian state of the Amazonas. Diffusion axes of disease were mapped and statistically analyzed in association of rowboats flows in the state. The results described an association of diffusion and evolution of the COVID-19 cases with the fluvial transport of viruses by mobility of the rowboats flows, which contributed to the internalization of disease in the state.